摘要
YN87448 virus strain was isolated from a fevered female patient (52 years old ) in Yunnan Province in 1986, and was identified as a member of Alphavirus using serological method. One primer was designed from common hairpin conserved region of Alphavirus. Two fragments were amplified by single primer differential RT PCR, and they were cloned into pGEM T vector. Sequences were determined, and then analyzed by Software and GenBank. Results showed that the 1118pb long fragment was highly homologous to the sequence of Sindbis like virus S.A.A.R86. The homogeneity of the 1118bp fragment between YN87448 virus strain and S.A.A.R86 virus strain was 98%. Single primer differential RT PCR is a useful method with simple, economic, and practical value for Alphavirus identification.
YN87448 virus strain was isolated from a fevered female patient (52 years old ) in Yunnan Province in 1986, and was identified as a member of Alphavirus using serological method. One primer was designed from common hairpin conserved region of Alphavirus. Two fragments were amplified by single primer differential RT PCR, and they were cloned into pGEM T vector. Sequences were determined, and then analyzed by Software and GenBank. Results showed that the 1118pb long fragment was highly homologous to the sequence of Sindbis like virus S.A.A.R86. The homogeneity of the 1118bp fragment between YN87448 virus strain and S.A.A.R86 virus strain was 98%. Single primer differential RT PCR is a useful method with simple, economic, and practical value for Alphavirus identification.
出处
《病毒学报》
CAS
CSCD
北大核心
2000年第1期59-60,共2页
Chinese Journal of Virology
基金
国家自然科学基金!( 3 982 3 0 0 2 )
国家优秀重点实验室基金资助
