摘要
选用经筛选的 2 0个短序列引物 ( 1 0 nt)和随机抽取的 2个随机长序列引物 ,对激光诱导产生的无核沙田柚 (处理组 )及未经激光处理的有核沙田柚 (对照组 )进行了 RAPD(随机扩增多态性 DNA)分析。结果有6个引物扩增出了共 2 0条多态性片段 ,从而表明在沙田柚的处理组与对照组之间 ,存在着 DNA分子水平上差异 ,也就是说 ,已经引起了沙田柚后代基因组的显著变异 。
Twenty short arbitrary primers(10nt)and two long ones(20nt) were used in random amplified polymorphic DNA(RAPD) analysis for different strains of Shatian Pumelo. Among them, six primers could generate twenty polymorphic bands. The result was shown that there were some genetic variations among the Shatian Pumelo stains irradiated by laser and nonirradiated with RAPD method. And the analysis of PAPD demonstrated that the laser irradiation can induce the genetic variation of Shatian Pumelo.
出处
《激光生物学报》
CAS
CSCD
2000年第1期4-7,共4页
Acta Laser Biology Sinica
基金
国家自然科学基金!资助项目 (编号 :695 680 0 1)
国家基金广西匹配项目!(编号 :桂科配 962 2 0 15 )
