离子注入水稻愈伤组织提高农杆菌转化效率的初步研究

Implantation Raise Transformation Efficiency of Rice Callus Mediated by Agrobacterium tumefaciens

通过 GUS报告基因表达的组织化学染色分析 ,我们发现低能氮离子束注入水稻愈伤组织可显著提高根癌农杆菌介导转基因效率 ,这为克服单子叶植物对根癌农杆菌不敏感性、提高遗传转化效率的研究提供了一条新的途径。

In research of Agrobacterium tumefaciens mediated transformation of rice callus,histochemical analysis of GUS reporter gene showed that rice callus pretreated with ions implantation were easier to be transformed by Agrobacterium tumefaciens.This provide a new way to overcome the recalcitrance of cereal plants to Agrobacterium tumefaciens