阻塞性肺气肿大鼠血清和支气管肺泡灌洗液中MMP-9、TNF-a、TGF-β1、IL-10水平变化

Levels of MMP-9, TNF-α TGF-β1 and IL-10 in serum and bronchoalveolar lavage fluid from rats with obstructive emphysema

目的探讨MMP-9、TNF-a、TGF-β1、IL-10在慢性阻塞性肺疾病(COPD)发病机制中的作用。方法 40只Wistar大鼠随机分为单纯吸烟组(S组)、单纯肺炎克雷伯杆菌感染组(I组)、吸烟加肺炎克雷伯杆菌感染组(E组)、对照组(C组)。16周后处死动物,HE染色观察肺脏病理变化,ELISA检测血清和支气管肺泡灌洗液(BALF)中MMP-9、TNF-a、TGF-?1、IL-10的水平。结果 E组肺组织病理改变明显,S组和I组均较轻微。E、S、组的BALF中MMP-9、TNF-a、TGF-β1及IL-10水平均明显高于C组(P<0.01),其中E组最明显;I组的BALF中MMP-9和TGF-β1水平高于S组(P<0.01)。E组的血清MMP-9水平高于S、I和C组,但TNF-a水平降低(P<0.01);E、S、I组的血清TGF-β1水平高于C组,但IL-10水平降低(P<0.01)。结论 MMP-9、TNF-a、TGF-β1及IL-10均参与COPD的发病。

Objective To investigate the role of MMP-9, TNF-α TGF-β1 and IL-10 in the pathogenesis of chronic obstructive pulmonary disease （COPD）. Methods Forty Wistar rats were randomly divided into smoking group （S group ）, Klebsiella pneumoniae infection group （I group）, smoking and Klebsiella pneumoniae group （E group） and control group （C group）. All rats were sacrificed after 16 weeks, and pulmonary pathology and levels of MMP-9, TNF-α TGF-β1 and IL-10 in serum and bronchoalveolar lavage fluid （BALF） were detected by HE stain and ELISA, respectively. Results The change of pulmonary pathology was obvious in E group, but mild in S and I groups. BALF levels of MMP-9, TNF-α TGF-β1 and IL-10 levels in E, S and I groups were superior to those in C group （P0.01）, especially in E group; BALF levels of MMP-9 and TGF-β1 were higher in I group than those in S group （P0.01）. Compared with S, I and C groups, serum MMP-9 contents increased but TNF-α decreased in E group （P0.01）; serum TGF-β1 concentrations increased but IL-10 decreased in E, S and I groups compared with C group （P0.01）. Conclusion MMP-9, TNF-α TGF-β1 and IL-10 are all involved in the pathogenesis of COPD.