湖南地区汉族人群mir-499基因多态性与鼻咽癌发生发展的关联性研究

Association of mir-499 gene polymorphism with progression of nasopharyngeal carcinoma in Hunan Han population

目的研究mir-499(rs3746444)位点多态性与鼻咽癌发病风险及临床病理因素的关系。方法采用病例-对照研究方法,用PCR-RFLP检测152例湖南地区汉族鼻咽癌患者(病例组)及性别、年龄匹配的197例正常人(对照组)mir-499(rs3746444)多态性分布,分析其与鼻咽癌风险及临床病理因素的关系。结果 mir-499基因多态性位点rs3746444的基因型和等位基因分布在病例组和对照组中差异无统计学意义(P>0.05)。T3、T4期鼻咽癌患者携带CC基因型的比例低于T1、T2期患者(P=0.027)。结论 mir-499基因多态性与湖南地区汉族人鼻咽癌的疾病发生无关,与鼻咽癌的疾病进展有关。

Objective To investigate the relationship between mir-499 （rs3746444） gene polymorphism and susceptibility and clinicopathologic factors of nasopharyngeal carcinoma （NPC）. Methods The mir-499 （rs3746444） gene polymorphism was detected by PCR-RFLP in 152 NPC cases and 197 gender and age-matched controls in Hunan Han people, and the relationship between its SNP and NPC susceptibility and clinicopathologic factors was analyzed. Results The genotype and allele frequency of mir-499 （rs3746444） showed no difference between both groups （P0.05）. The frequency of CC genotype was lower in T3 and T4 stages of NPC than that in T1 and T2 stages （P=0.027）. Conclusion Polymorphism of mir-499 （rs3746444） may be related to the progression rather than development of NPC in Hunan Han people.