大豆蚜基因组DNA四种提取方法的比较

Comparison of Four Methods for Genomic DNA Extraction from Aphis glycines

以大豆蚜(Aphis glycines)为材料,比较分析了氯仿-异戊醇抽提法、盐析法、KAc法及试剂盒法4种基因组DNA提取方法。结果表明:在提取大豆蚜基因组DNA的纯度上,氯仿-异戊醇抽提法和盐析法较高;提取所用时间由短到长依次为试剂盒提取法、盐析法、氯仿-异戊醇抽提法和KAc法。盐析法能够更快速有效地提取大豆蚜基因组DNA,是一种值得推广应用的基因组DNA提取方法,另外3种方法也有一定的优势,应根据试验条件和要求选择相应的基因组DNA提取方法。

Using Aph/s glycines as material ,four genomic DNA extraction method including chloroform-isoamyl alcohol extraction, salting-out method, KAc and genomic DNA kit method were compared and analyzed. The purity of genomic DNA from chloroform-isoamyl alcohol extraction and salting-out method was better than the other two methods. And the total time of extraction in ascending order was genomic DNA kit method, salting-out method, the chloroform-isoamyl alcohol extraction and KAc. Salting-out method acted more quickly and effectively, the other three methods also had some advantages, therefore, the corresponding methods for genomic DNA extraction should be chosen according to the test conditions.