休眠期大豆胞囊线虫体内关键酶活性变化

Changes of Related Enzyme Activities in Dormant Soybean Cyst Nematode,Heterodera glycines

大豆胞囊线虫(Heterodera glycines,SCN)病是一种重要的大豆病害,以休眠胞囊越冬。为明确休眠期SCN的生理代谢变化,采用分光光度仪测定了休眠期SCN体内几种关键酶活性。结果表明:海藻糖酶(trehalase,TRE)、糖原磷酸化酶(glycogen phoshorylase,GP)、山梨醇脱氢酶(NAD-sorbitol dehydrogenase,NAD-SDH)、磷酸果糖激酶(phospho-fructokinase,PFK)、丙酮酸激酶(pyruvate,PK)和果糖1,6-二磷酸羧醛酶(fructosel,6-bisphosphotase,FBP)均与SCN休眠密切相关,进入休眠SCN体内海藻糖酶活性迅速升高,糖原磷酸化酶活性也显著升高,有利于海藻糖所需碳源的供给,而磷酸果糖激酶、丙酮酸激酶、果糖1,6-二磷酸羧醛酶和山梨醇脱氢酶4种酶活性均显著降低,休眠解除后活性回升。

Soybean cyst nematode （SCN） disease （ Heterodera glycines）, which is a major disease of soybean, overwinters in its dormant stage. In order to find out the physiological metabolism of dormant SCN, the activities of dormancy-related enzymes, including trehalase （ TRE ）, glycogen phoshorylase （ GP）, NAD-sorbitol dehydrogenase （ NAD-SDH ）, phosphofructokinase （ PFK ）, pyruvate（PK） and fructose. 1,6-bisphosphotase（FBP） ,were assayed in dormant SCN during different dormancy stage by spectrophotometer. The result showed that activities of these enzymes were closely related to dormancy of SCN, significantly increasing of TRE and GP activities when SCN entered into dormant stage,favored to supply of carbon source needed by TRE. But the activity of PFK, PK, FBP and NAD-SDH in dormant SCN were obviously lower than active SCN, then went up after dormancy broken.