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DNA测序技术进展及其应用 被引量：1

The Progress and Applications of DNA Sequencing Techniques
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摘要 自1977年以Sanger法为代表的第一代测序技术确立以来，在过去的三十年里，DNA测序技术已经取得了重要的进展。随着大规模高通量新一代测序技术的发明，各种基因组项目已经可以在个人实验室中完成，更是大大加速了生物学领域的研究步伐。本文介绍了目前所应用的测序技术及其应用进展，并对其发展趋势进行了展望。 Over the past three decades, remarkable DNA sequencing technological innovations has emerged since Sanger- based sequencing technology established in 1977. With the development of next-generation high-throughput DNA sequencing techniques, the genome sequencing is feasible in individual laboratories which has dramatically accelerated biological research. The progress and applications of current sequencing techniques and their future perspectives will be discussed here.
作者 刘佶 何宏 刘蓬蓬
机构地区 青岛大学医学院附属医院
出处 《生命科学仪器》 2013年第4期21-25,共5页 Life Science Instruments
关键词 DNA测序技术 Sanger法测序 SOLiD~序 Sanger-based sequencing technology next-generation sequencing SOLID sequencing
分类号 Q523.8 [生物学—生物化学]
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参考文献22

  • 1Sanger, F. et al. Nucleotide sequence of bacteriophage phi X174 DNA. Nature 265, 687-95 (1977).
  • 2Sanger, F., Nicklen, S. & Coulson, A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A 74, 5463-7 (1977).
  • 3Maxam, A. M. & Gilbert, W. A new method for sequencing DNA. Proc Natl Acad Sci U S A 74, 560-4 (1977).
  • 4Ansorge, W. J. Next-generation DNA sequencing techniques. N Biotechnol 25, 195-203 (2009).
  • 5Margulies, M. et al. Genome sequencing in microfabricated high-density picohtre reactors. Nature 437, 376-80 (2005).
  • 6Fedurco, M., Romieu, A., Williams, S., Lawrence, I. & Turcatti, G. BTA, a novel reagent for DNA attachment on glass and efficient generation of solid-phase amplified DNA colonies. Nucleic Acids Res 34, e22 (2006).
  • 7Turcatti, G., Romieu, A., Fedurco, M. & Tairi, A. P. A new class of cleavable fluorescent nucleotides: synthesis and optimization as reversible terminators for DNA sequencing by synthesis. Nucleic Acids Res 36, e25 (2008).
  • 8Shendure, J. et al. Accurate multiplex polony sequencing of an evolved bacterial genome. Science 309, 1728-32 (2005).
  • 9Shendure, J. & Ji, H. Next-generation DNA sequencing. Nat Biotechnol 26, 1135-1145 (2008).
  • 10Sultan, M. et al. A global view of gene activity and alternative splicing by deep sequencing of the human transcriptome. Science 321,956-60 (2008).

同被引文献43

  • 1张晓丹,武海萍,周国华.焦测序技术及其在遗传分析中的应用[J].分析化学,2006,34(4):582-586. 被引量：19
  • 2Whitfeld PR. A method for the determination of nucle- otide sequencein polyribonucleotides. Biochem J, 1954, 58:390-396.
  • 3Maxam AM, Gilbert WA. New method for sequencing DNA. Proc Nail Acad Sci USA, 1977, 74:560-564.
  • 4Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Nail Acad Sci USA, 1977, 74:5463-5467.
  • 5Martin GR. The roles of FGFs in the early development of vertebrate limbs. Genes Dev, 1998, 12:1571-1586.
  • 6MaxamAM, GilbertW. Sequencingend-labeled DNA with basespecific chemical cleavages. Methods Enzymol, 1980, 65" 499-560.
  • 7Adams MD, Celniker SE, Holt RA, et al. The genome sequence of Drosophila melanogaster. Science, 2000, 287:2185-2195.
  • 8Zubritsky E. How analytical chemists saved the human genome project. Anal Chem, 2002, 74:23-26.
  • 9Metzker ML. Sequencing technologies-the next genera- tion. Nat Rev Genet, 2010, 11:31-46.
  • 10Margulies M, Egholm M, Altman WE, et al. Genome sequencing in microfabricated high-density picoolitre reactors. Nature, 2005, 437:376-380.

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生命科学仪器
2013年 第4期

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