和厚朴酚对小鼠四氯化碳肝损伤的保护作用

Protective effect of honokiol on liver injury induced by carbon tetrachloride in mice

目的:探讨和厚朴酚(HNK)对四氯化碳(CCl4)诱导的肝损伤的保护作用。方法:将50只小鼠随机分成HNK高、中、低剂量组(分别以20.0、2.0、0.2mg/kg剂量皮下注射HNK注射液),正常组和模型组(注射等量生理盐水)5组。给药7d后,模型组和HNK各剂量组腹腔注射含CCl4的大豆油溶液,正常组注射等体积大豆油溶液。24h后,摘除眼球取血,解剖肝脏,观察小鼠肝脏的病理学变化,应用生化分析仪测定血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)、碱性磷酸酶(ALP)、血糖(Glu)含量,Real-timePCR法分析血清中ALT、AST、ALP和肝脏中肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)、干扰素-γ(IFN-γ)mRNA的表达。结果:HE染色结果显示,正常小鼠肝组织结构完整,肝细胞呈索状结构排列,肝细胞分界清晰,未见脂肪变性和炎性细胞浸润。模型组小鼠肝组织肝索排列紊乱,中央静脉变形,肝细胞肿胀并呈泡状脂肪变性,伴淋巴细胞和中性粒细胞浸润。HNK各剂量组上述病理变化均有不同程度减轻,尤以HNK高剂量组改善较明显。与模型组相比,HNK处理后,小鼠血清中ALT、AST、ALP含量和mRNA水平及Glu浓度均明显降低(F分别为274.620,1121.720;355.870,69.430;294.280,35.330;75.770;P均<0.001);同时,肝脏组织中TNF-α、IL-6和IFN-γmRNA的表达明显降低(F分别为1165.950,69.460,4787.190;P均<0.001)。结论:HNK对CCl4诱导的小鼠急性肝损伤有保护作用。

Aim： To observe the protective effect of honokiol on liver injury induced by carbon tetrachloride （CCl4） in mice.Methods： Fifty mice were randomly divided into five groups.The HNK high,middle and low dose groups were injected honokiol solution at a concentration of 20.0,2.0,and 0.2 mg/kg,respectively,and the normal group and model group were injected normal saline,respectively.After 7 d,animals in the model group and HNK groups were injected 10 mL/kg CCl4,others were given soybean oil.Histological analysis of liver was performed by hematoxylin and eosin.The contents of serum alanine aminotransferase（ALT） ,aspartate aminotransferase（AST） ,alkaline phosphatase（ALP） and glucose（Glu） were determined through automatic biochemistry analyzer.The mRNA levels of ALT,AST,ALP,tumor necrosis factor-α （TNF-α） ,interleukin-6（IL-6） and interferon-γ（INF-γ） in livers were analyzed by real-time PCR.Results： Compared with the normal group,the liver sections of model group showed destructive alterations in the parenchyma,extensive fatty changes,disarrangement of hepatic cords and central veins,centrilobular necrosis,focal necrosis.The content of ALT, AST,ALP,and Glu in HNK groups were lessened in comparison with control group （F=274.620,1 121.720; 355.870, 69.430; 294.280,35.330; 75.770; P0.001） .The mRNA expression of TNF-α,IL-6 and IFN-γ in liver was decreased in comparison with control group（F=1 165.950,69.460,4 787.190; P0.001） significantly.Conclusion： The HKK canplay a protecting role against liver injury induced by CCl4.