慢性阻塞性肺疾病患者肺组织中Bach1和γ-GCS的表达

Expressions of Bach1 and γ-GCS in lung tissue of patients with chronic obstructive pulmonary disease

目的:研究慢性阻塞性肺疾病(COPD)患者肺组织中BTB-CNC异体同源体1(Bach1)和γ-谷氨酰半胱氨酸合成酶(γ-GCS)的表达。方法:收集因肺肿瘤行肺叶切除术患者的肺组织标本,诊断符合COPD者入选COPD组(20例),不符合者入选对照组(20例)。于远离病灶处取材,分别采用原位杂交和免疫组化方法检测肺组织中Bach1、γ-GCSmRNA和蛋白的表达。结果:COPD组患者肺组织中Bach1、γ-GCSmRNA和蛋白的表达均较对照组增强(t分别为9.946,14.573,14.134,14.365,P均<0.001)。COPD患者肺组织中Bach1蛋白与γ-GCSmRNA、蛋白表达均呈直线负相关(r为-0.834,-0.815,P<0.001)。结论:Bach1可能通过负调控γ-GCS基因的表达,从而在COPD的氧化/抗氧化调节过程中发挥重要作用。

Aim： To investigate the expressions of BTB and CNC homology 1 （Bach1） and γ-glutamylcysteine synthetase（γ-GCS） in lung tissue of the patients with chronic obstructive pulmonary disease（COPD） .Methods： Lung specimens were obtained from 40 patients who underwent lobectomy for lung tumor,of whom 20 had concurrent COPD and 20 without COPD.The samples were taken from the areas remote to the lesion,and in situ hybridization and immunohistochemistry were used to detect the mRNA and protein expressions of Bach1 and γ-GCS,respectively.Results： The mRNA and protein expression levels of Bach1 and γ-GCS in COPD group were significantly higher than those in the control group（t was 9.946,14.573,14.134,14.365,respectively,P0.001） .The Bach1 protein expression level was negatively correlated with those of γ-GCS mRNA and protein （r=0.834, 0.815,P0.001） in COPD group.Conclusion： Bach1 may negatively regulate the expression of γ-GCS gene,and play an important role in the process of oxidation/antioxidation of COPD.