草鱼免疫器官和血液的六种同工酶生化与遗传特性分析

Analysis of biological and genetic characteristics of six isozymes of blood and immune organs from grass carp (Ctenopharyngodon idellus Cuvier et Valencinnes)

采用聚丙烯酰胺凝胶电泳技术,对草鱼(CtenopharyngodonidellusCuvieretValencinnes)血液及不同免疫器官中六种同工酶系统(乳酸脱氢酶、醇脱氢酶、苹果酸脱氢酶、3 磷酸甘油脱氢酶、酯酶和过氧化物酶)的分布特性、活性含量、酶谱表型及迁移特征进行了分析,并就不同酶谱特征及其相应基因座的组合表达方式及特点作了讨论。结果表明,对同一种遗传型的草鱼来说,不同免疫器官的同工酶区带的表达与免疫器官的遗传多样性和组织特异性有关,这进一步证实了其特征性表达的同工酶机能的专一性。

Six isozymes, i.e., lactate dehydrogenase (LDH), alcohol dehydrogenase (ADH), malate dehydrogenase (MDH), 3 glycerophosphate dehydrogenase (3 GPDH), esterase (EST), peroxidase (POX), extracted from the blood and immune organs of grass carp ( Ctenopharyngodon idellus Cuvier et Valencinnes), were analyzed with vertical slab poly acrylamid gel electrophoresis and by specific cytochemical staining. The isozymic distribution traits, relative activities, phenotypes, and the mobile characteristics were determined, and the possible relationships between the isozyme pattern and the combinatorial express way for the relevant loca were disscused. The results indicated that it was related to its genetic diversities and the tissue specific characters that the different immune organs expressed the different isozyme's bands for the grass carp in the same genetic type,and validated further the undivided traits of the isozymic function characteristically.