正加速度(+Gz)暴露对冠脉不同程度狭窄猪血浆CK-MB和IL-6变化的影响研究

Study on Changes of Plasma Creatine Kinase Isozyme and Interleuki-6 Levels in Swines with Different Coronary Artery Stenosis after Positive Acceleration (+Gz) Exposure

目的:观察正加速度(+Gz)暴露处理后不同冠脉狭窄程度猪的血浆肌酸激酶同工酶(CK-MB)和白介素-6(IL-6)水平的变化特点,探讨心脏的危害,评估飞行员的飞行。方法:(1)20头巴马小型猪,在胸腔镜直视下手术丝线永久性结扎左前降支近端建立轻度(狭窄程度20-49%)、中度(狭窄程度50-69%)和重度(狭窄程度≥70%)狭窄的冠状动脉狭窄模型;(2)各组小型猪进行+Gz暴露,分别观察其最大+Gz加速度耐受值;(3)各组小型猪分别在其最大+Gz加速度暴露处理前后1 min留取静脉血和分离血浆,测定CK-MB及IL-6的含量。结果:(1)各组小型猪最大+Gz加速度耐受值分别为:①正常对照组:+8 Gz;②轻度狭窄组:+8Gz;③中度狭窄组:+6 Gz;④重度狭窄组:+5 Gz;(2)最大耐受+Gz加速度暴露对各组小型猪血浆CK-MB及IL-6的影响差异有统计学意义(P<0.01),同时,在分别经过最大耐受+Gz加速度暴露处理后,冠脉中度和重度狭窄模型猪血浆CK-MB和IL-6的浓度较正常对照组均显著升高(P<0.01),轻度狭窄组则升高不明显(P>0.05)。结论:(1)+Gz暴露对冠脉不同程度狭窄小型猪血浆CK-MB和IL-6水平的影响不同,狭窄程度越重,影响越大。(2)轻度狭窄模型猪在+Gz暴露下血浆CK-MB和IL-6的浓度改变与正常对照组相似,提示冠脉轻度狭窄的飞行员可以继续飞行。

Objective： To observe the characteristics of the changes of plasma CK-MB and interleuki-6 levels in swines with different coronary artery stenosis after ＋Gz exposure,so as to explore the heart of the hazards,assess the pilot＇s flight.Methods：（1）Of 20 Bama miniature swines,the proximal left anterior descending branch（LAD） was ligated permanently by using silk suture under direct vision of the thoracoscope to establish different coronary artery stenosis models--the mild,the moderate and the severe--with stenosis degree of the LAD 20-49%,50-69%,and ≥70% respectively.（2）＋Gz exposure was performed and values of the maximum ＋Gz acceleration tolerance were observed in each group;（3）1 minute before and after the exposure of the maximum ＋Gz acceleration tolerance in each group,venous blood was collected.Then,plasma were separated and CK-MB,IL-6 were measured.Results：（1） Values of the maximum ＋Gz acceleration tolerance in each group are as follows： ①The normal control group： ＋8 Gz;②The mild stenosis group：＋8 Gz;③The moderate stenosis group：＋6 Gz;④The severe stenosis group： ＋5 Gz;（2） The effects of the exposure of the maximum ＋Gz acceleration tolerance on plasma CK-MB and IL-6 content were significant（P0.01）.Meanwhile,compared with the normal control group after the exposure of the maximum ＋Gz acceleration tolerance,plasma C-reactive protein and endothelin-1 levels in the moderate and the severe groups were significantly raised,but the mild was not.Conclusions： 1.The effect of ＋Gz exposure on plasma CK-MB and IL-6 levels in swines with different coronary artery stenosis is different-the worse the stenosis degree,the bigger the effect;2.Changes of plasma CK-MB and IL-6 levels in the normal control group are similar with those in the mild after ＋Gz exposure,the mild stenosis coronary pilots can continue flying.