腹膜间皮细胞与聚羟基乙酸的细胞相容性研究

Experimental Research on Cell Biocompatibility of Polyglycolic Acids with Peritoneal Mesothelial Cell

目的:研究腹膜间皮细胞(Peritoneal mesothelial cell,PMC)与可吸收生物材料聚羟基乙酸(Polyglycolicacids,PGA)的相容性,为组织工程化尿道的构建奠定基础。方法:采用酶消化法,从SD大鼠腹腔中分离出PMC,常规传代,取第2代细胞与PGA混合培养,形成细胞-生物材料复合物。每两天换液1次,观察细胞形态和黏附生长状况。体外培养1周后,行扫描电镜观察和RT-PCR检测。结果:复合物体外培养1周后,PMC能成功黏附在PGA支架上,并分泌大量细胞外基质。RT-PCR提示CK-AE1/AE3和Vimentin均呈阳性表达,PMC表型未发生改变。结论:PMC与PGA有良好的细胞相容性,PGA可作为组织工程化腹膜样组织的支架材料。

Objective： To investigate the cell biocompatibility of polyglycolic acids（PGA） with peritoneal mesothelial cell（PMC） in vitro.Methods： PMCs were isolated by enzyme digestion from rat peritoneal and multiplied in culture.PMCs of the second passage were collected and seeded onto PGA unwoven fibers.The cell-biomaterials complex was cultured in DMEM medium,and the medium was changed every 2 days.After the first week,the PMC-PGA sheets were cut off for scanning electron microscope examination and RT-PCR analysis.Results： At the first week,PMCs adhered the PGA scaffolds and secreted much of extracellular matrix proteins.RT-PCR revealed the expression of Vimentin and CK-AE1/AE3 in gene.Conclusion： PGA presented a good biocompatibility with PMCs and could be used as tissue-engineered peritoneum-like tissue.