脑脊液5-羟色胺浓度对大鼠中枢性睡眠呼吸暂停的影响

Serotonin concentration of cerebrospinal fluid effect on rats with sleep apnea

目的探讨脑脊液5-羟色胺(5-HT)浓度对中枢性睡眠呼吸暂停的影响。方法对8只成年雄性SD大鼠进行连续3d的睡眠呼吸监测,第1天为空白(A组),第2天给予新鲜脑脊液(ACSF)(B组),第3天给予5-HT(C组)。记录呼吸暂停指数、睡眠效率、各期睡眠时间,进行重复测量资料的方差分析。结果 (1)C组非快速眼动期呼吸暂停指数较A组和B组明显降低(P<0.05)。(2)C组快速眼动期(REM期)叹息后呼吸暂停指数较A组明显降低(P=0.019)。(3)三组在REM期自发性呼吸暂停指数、睡眠效率、NREM期睡眠时间上差异无统计学意义。(4)C组REM期睡眠时间较A组明显减少(P=0.038)。结论提高脑脊液5-HT浓度可改善SD大鼠NREM期睡眠呼吸暂停及REM期叹气后呼吸暂停,对SD大鼠睡眠效率及NREM期睡眠时间无影响。

Objective To investigate cerebrospinal fluid concentration of serotonin within the influence of central sleep apnea. Methods Carried out on 8 adult male SD rats sleep apnea monitoring for 3 consecutive days, first day for the blank （group A）, the second day to artificial cerebrospinal fluid （ACSF） （group B）, the third day to 5-HT （group C）. Record REM-PSAI, sleep efficiency, every period of sleep time and compare the result from repeated measurement data analysis of variance. Results Non-em stage apnea index of group C was significantly lower in group A and group B（P〈0.05）. The rapid eye movement （REM） sigh of group C after apnea index （REM-PSAI） was significantly lower in group A （P〈0. 019）. The three groups in the REM stage of spontaneous apnea index （REM-SPAI）, sleep efficiency, phase NREM sleep there were no statistically significant differences in time. The REM phase of sleep time of group C compared with group A decreased significantly （P=0. 038） Conclusion Improve cerebrospinal fluid concentration of 5-HT can improve SD rats stage NREM and REM sleep apnea after sighing apnea, sleep efficiency of SD rats and NREM sleep time had no effect.