银杏叶片对大鼠肝纤维化的疗效及肝脏过氧化物酶体增殖物激活受体表达的影响

Effects of the Ginkgo biloba on PPAR Expression in CCl4-induced hepatic fibrosis in rats

目的探讨银杏叶片对大鼠肝纤维化的疗效及部分可能机制。方法将造模成功后的20只WISTAR大鼠随机分为治疗组及自然恢复组,治疗组正常进食水且每日以银杏叶片灌胃,空白对照组及自然恢复组正常进食水,同时每日以等量自来水灌胃。2个月后留取血清、肝组织,测定其血清透明质酸(HA)含量及肝组织中羟脯氨酸(Hpy)含量,行组织病理学检查评价肝纤维化形成情况,并行免疫组化检查观察过氧化物酶体增殖物激活受体(PPAR-γ)的表达情况。结果自然恢复组及治疗组大鼠血清HA及肝组织Hpy浓度与空白对照组相比较均升高(P<0.05),其中自然恢复组较治疗组升高明显(P<0.05)。免疫组化结果显示PPAR-γ在空白对照组表达最显著,自然恢复组及治疗组较空白对照组表达均下调(P<0.05),而与自然恢复组相比,治疗组大鼠肝脏PPAR-γ表达水平明显上调(P<0.05)。结论银杏制剂治疗肝纤维化的机制之一可能为上调PPAR-γ水平的表达。

Objective To investigate the therapeutic effect of ginkgo leaves on hepatic fibrosis so as to find a theoretical basis for the clinical aplication of ginkgo leaves preparation. Methods Fifty male Wistar rats were randomly assigned into normal control group （n=10） and model group （n=40）. All the rats in model group were subcutaneously given CCL4 injection, high-fat and low-protein diet and alcohol to establish hepatic fibrosis models. 8 week later three rats were sacrificed to make sure the success of hepatic fibrosis model. The survived 20 rats in model group were again randomly divided in to 2 groups which were spontaneous recovery group and treated group. Rats in treated-group were conventionally fed and perfused with extracts of ginkgo leaves every day, while the rats in normal con- trol group and spontaneous group were conventionally fed and per{used with water. 8 weeks later, the liver index, levels of serum hyaluronic acid （HA） and liver hydroxyproline （Hyp） were measured, and the degree of hepatic fibrosis was estimated. The expression of peroxisome proliferator-activated re- ceptors γ （PPAR-γ） was detected by immunohistochemistry. Results Compared with normal control group, the serum HA and the liver Hyp increased in spontaneous recover group and the treated group （P〈0.05） , while the serum HA and the liver Hyp in treated group decreased compared with sponta- neous recover group （P〈0.05）. The expression of PPAR-γ was highest in normal control group. Compared with normal control group, the expression of PPAR-γ decreased in spontaneous recovergroup and the treated group（P〈0.05）, while compared with spontaneous recover group, the expres- sion of PPAR-γ in treated group increased remark（P〈0.05）. Conclusion The extracts of ginkgo may palliate the hepatic fibrosis by regulating the expression of PPAR-γ.