摘要
为揭示牛MHC-DRB3上游调控区多态对其表达调控作用,利用PCR-SSCP和DNA序列测定技术分析并建立BoLA-DRB3*exon2和BoLA-DRB3 URR之间的连锁关系。结合不同近端调控区与转录因子结合模式预测结果,选择3种不同上游调控序列的连锁组合,应用实时定量RT-PCR技术对牛MHC-DRB3基因mRNA相对表达量进行了验证分析。结果表明,上游调控区基因型为H17H17的BoLA-DRB3基因mRNA相对表达量显著高于(P<0.01)基因型H1H1及H5H6。初步证明上游调控区中的多态会引起结构基因表达量的变化。
To study the regulation of upstream regulatory region polymorphisms on BoLA-DRB3 gene expression,PCR-SSCP and DNA sequencing were used to identify the linkage relationship between BoLA-DRB3＊exon2 and BoLA-DRB3 URR.Combined with the predicted results of binding mode between different URR and transcription factor,three linkage types were selected to detect the BoLA-DRB3 mRNA levels by relative Real-time PCR experiments.The results showed that the expression abundances of BoLA-DRB3 varied in different linkage types.The expression level of mRNA of BoLA-DRB3 gene linked with H17H17 was significant higher than H1H1 and H5H6（P0.01）.This preliminary proved that the polymorphism of upstream regulatory region can cause the expression change of BoLA-DRB3 structural gene.
出处
《华北农学报》
CSCD
北大核心
2013年第4期19-22,共4页
Acta Agriculturae Boreali-Sinica
基金
河北省自然科学基金项目(C2012301011)
河北省农林科学院财政项目(2010055008)
