利用染色体单片段代换系定位水稻穗粒数QTL

Identification of Grain Number Quantitative Trait Loci with Single Segment Substituted Lines in Rice

以85个单片段代换系为材料,其受体亲本为广陆矮4号,供体亲本为日本晴。通过单因素方差分析和Dunnett's多重比较,分析单片段代换系与受体亲本之间穗粒数的差异,对代换片段上的穗粒数QTL进行鉴定。以P≤0.001为阈值共检测到27个穗粒数QTLs,分布于除第10染色体外的其他11条染色体上。其中,2个QTLs的加性效应表现为减效作用,另外25个穗粒数QTLs的加性效应表现为增效作用。其加性效应值的变化范围为-37.5～96.9,加性效应百分率的变化范围为-29.07%～88.57%。为进一步发掘和利用新的水稻穗粒数QTL奠定基础。

Grain number is one of the important components of rice yield which is a typical quantitative trait and can easily be influenced by genetic background and environmental factors.Single segment substitution lines（SSSLs） can effectively reduce the interference of the genetic background.We developed a set of genome-wide SSSLs carrying Nipponbare introgression segments in the background of Guangluai 4.Quantitative trail loci（QTL） analysis involved one-way ANOVA and Dunnett′s test for comparing SSSLs with the recurrent parent Guangluai 4（P≤0.001）.We detected 27 QTLs for grain number distributed on 11 rice chromosomes,except chromosome 10,of which 2 had negative effect and the others positive effect.The additive effect of these QTLs ranged from-37.5 to 96.6,with additive effect percentages-29.07% to 88.57%.These results provide useful information for exploring and using new grain number related resources.