摘要
为了提高玉米中赖氨酸含量,利用基因枪技术将Sb401基因导入玉米杂交组合HiⅡAB的愈伤组织中,在添加Bialaphos浓度为1.5,3.0 mol/L的选择培养基上进行2次筛选,然后分化再生植株,对所获得的植株进行PCR检测和免疫试纸条检测。结果显示,经过2次筛选获得58株再生植株,PCR检测呈阳性的植株有38株,将PCR阳性植株再通过免疫试纸条检测,确定bar蛋白得到表达的植株有31株,结果表明,共获得31株含有目的基因的玉米植株。
The purpose of this study was to improve the lysine content in corn,Sb401 gene was introdued into immature embryo callus using particle bombardment.Regeneration into plants by selection for 1.5,3.0 mol/L bialaphos resistance.PCR detection in the obtained by plants and protein dipsticks testing.The results show that 38 transgenic plants were identified by PCR from 58 regenerated transformed plants obtained by selection for bialaphos resistance,31 transgenic plants was identified by test trip.It turned out that 31 transgenic plants has introduced target gene.
出处
《华北农学报》
CSCD
北大核心
2013年第4期66-68,共3页
Acta Agriculturae Boreali-Sinica
基金
转基因玉米小麦大豆环境安全评价技术项目(2013ZX08011-003)
