阿巴嘎黑马染色体核型及G带分析

Karyotyping and G-banding Analysis of Abaga Horse

采用耳组织成纤维细胞培养方法及常规染色体标本制备技术,对阿巴嘎黑马的染色体核型及G带进行了分析。结果显示,阿巴嘎黑马染色体数目为2n=64,雄性核型为64,XY;雌性核型为64,XX。其中1～13号染色体为中或亚中着丝粒染色体,14～31号染色体为端着丝粒染色体,X染色体则为2对大亚中着丝粒染色体,Y染色体为最小的端着丝粒染色体。另外,阿巴嘎黑马染色体G带明暗相间,且每对染色体都有其独特的带纹特征,其中部分染色体的G带与已报道的阿拉伯马的G带特征有明显差异。本研究首次在国内报道了阿巴嘎黑马染色体核型及G带特征,这将为阿巴嘎黑马的细胞遗传学研究和疾病诊断提供资料。

In this paper,chromosomal karyotype of Abaga horse was studied by using the fibroblast cells culture method and conventional chromosome analysis techniques.The karyotyping of Abaga horse has been analyzed by standard and G-banding technique.The results showed that the diploid chromosome number of Abaga horse was 2n=64.The chromosome karyotypes of male and female horse were 64,XY and 64,XX.The chromosome 1-13 is metacentric-submetacentric autosome;chromosome 14-31 is acrocentric autosome.Chromosome X is the second longest submetacentric chromosome and Y is the smallest acrocentric chromosome.Moreover,each chromosome of Abaga horse has some unique patterns of its bands,and there are some differences with the reported Araba horse.This is the first report of G-banding karyotype of Abaga horse in China.The karyotype generated in this study will provide the fundamental information for equine cytogenetics and diagnosis of genetic disease in Arab horses.