家兔ATG16L1基因多态性与非特异性消化道紊乱易感性的研究

The Association between the Polymorphism of ATG16L1 and Non-specific Digestive Disorder in Rabbit

为了研究家兔ATG16L1基因对非特异性消化道紊乱的易感性,试验采用PCR-HRM技术,首次对家兔ATG16L1基因进行单核苷酸多态性(SNP)检测,同时结合低纤维群体结肠ATG16L1基因mRNA表达量进行关联分析。结果表明:ATG16L1基因存在1个SNP位点(c.1482 G>A)与NSDD的易感性相关联;c.1482 G>A位点A等位基因能增加NSDD的易感性(OR=1.43,95%置信区间:1.08～1.91,P<0.05);在隐性遗传模型中,AA基因型能增加NSDD的易感性(OR=1.69,95%置信区间:1.05～2.78,P<0.05);ATG16L1基因在结肠中的mRNA表达量随着炎症的加剧显著升高(P<0.05);AA基因型在整个低纤维诱导NSDD组中的表达水平最低(P<0.05)。这些结果均表明,ATG16L1基因与家兔NSDD的遗传易感性相关。

To study the susceptibility of ATG16L1 gene to non-specific digestive disorder（NSDD） in rabbit,single nucleotide polymorphism（SNP） of rabbit ATG16L1 gene was firstly detected by PCR-HRM and the association analysis of ATG16L1 gene mRNA expression in colon of fibre-deficient diet group was conducted.The results found that there was a SNP locus（c.1482 G〉A） in ATG16L1 gene and the SNP locus was associated with the susceptibility to NSDD;the allele A of c.1482 G〉A could increase the susceptibility（OR = 1.43,95% confidence interval：1.08-1.91,P〈0.05）;in recessive genetic model,the genotype AA could increase the susceptibility（OR = 1.69,95% confidence interval：1.05-2.78,P〈0.05）;along with the increasing severity of non-specific digestive disorder,the ATG16L1 gene mRNA expression in colon was gradually increased（P〈0.05）;genotype AA had the lowest mRNA expression in fibre-deficient diet group（P〈0.05）.These results showed that ATG16L1 gene was associated with the genetic susceptibility to NSDD in rabbit.