KLF6、KLF4及APC基因蛋白在结直肠癌中的表达和意义

Expression and significant of kruppel-like factor KLF6,KLF4and APC protein in human colorectal carcinoma and colorectal inflammatory tissues

目的研究转录因子KLF6、KLF4及APC基因在结直肠癌中的表达和意义。探讨它们在结直肠癌发生发展中的作用机制。方法应用免疫组化Envision法对58例结直肠癌组织、20例结直肠黏膜慢性炎症组织中的KLF6、KLF4及APC基因进行蛋白检测。结果KLF6、KLF4及APC蛋白在结直肠癌组织表达率分别为77.59%(45/58)、51.79%(30/58)、65.50%(38/58),低于在结直肠黏膜慢性炎症组织中的100%(20/20)、85%(17/20)、90%(18/20),差异有统计学意义(P<0.05);KLF6、KLF4及APC蛋白在结直肠癌组织中的表达与其浸润深度及预后有关(P<0.05),与淋巴结转移及Dukes分期无关(P>0.05)。结论KLF6、KLF4及APC基因可能在结直肠癌的发生发展中起重要作用。

Objective To investigate the expression and significance of KLF6, KLF4 and APC in human colorectal carcinoma, and explore its functional mechanisms in the oncogenesis and development of colorectal carcinoma. Methods By immunohistochemistry, expression patterns of KLF6, KLF4 and APC was studied in 58 cases of colorectal carcinoma and 20 cases of colorectal mucosa chronic inflam- matory tissues. Results The positive rates of KLF6, KLF4 and APC protein were 77.59% （45/58）, 51.79%（30/58）, 65.50 （38/58）in colorectal carcinoma, respectively, which was much lower than those 100% （20/20）, 85% （17/20）, 90~~ （18/20） in colorectal mucosa chronic inflammatory tissues （ P^0. 05）. The expression of KLF6, KLF4 and APC in colorectal carcinoma was correlate with theinvading and prognosis （P〈0.05）, but was not correlate with the metastasis of lymph nodes and Dukes stage（P^0.05）. Conclusion KLF6, KLF4 and APC may play an important role in the occur- rence and development of colorectal carcinoma.