嘉兴市霍乱弧菌分离株检测分析

Analysis of Vibrio cholerae isolates in Jiaxing

目的比较分析嘉兴市霍乱弧菌菌株的血清型、毒力基因和分子分型特征。方法采用血清学和分子生物学方法对近年来分离到13株霍乱弧菌菌株进行血清型分布、毒力基因(ctxA、ace、zot、tcpA、cri和rtxA)携带和ERIC-PCR分型研究。结果 13株霍乱弧菌菌株,2株为O139群霍乱弧菌,11株为O1群霍乱弧菌,其中小川型9株,稻叶型2株。2株O139群霍乱弧菌菌株携带3种毒力基因;11株O1群霍乱弧菌菌株,除1株携带4种毒力基因外,其余10株携带全部6种毒力基因。ERIC-PCR分型分析显示,不同的霍乱弧菌菌株之间ERIC-PCR型别表现出一定的遗传多样性。结论嘉兴市霍乱弧菌菌株大多携带多种毒力基因,而且来源多样,防控工作任重而道远。

Objective To analyze the serotype, virulence genes and molecular typing of Vibrio cholerae strains isolated in Jiaxing. Methods The serotype, virulence genes （ctxA, ace, zot, tcpA, cri and rtxA） and ERIC-PCR typing of 13 Vibrio cholerae strains isolated in recent years were studied by serological and molecu lar biological methods. Results Out of 13 Vibrio cholerae strains, 2 were identified as serogroup O139 and 11 were serogroup O1 which included 90gawa and 2 Inaba strains. 2 O139 strains carried 3 virulence genes. 10 O1 strains harbored all 6 virulence genes and only one carried 4. ERIC-PCR typing analysis showed that the ge netic diversity existed among different Vibrio cholerae strains. Conclusions Ma}ority of Vihrio cholerae strains isolated in Jiaxing carried multiple virulence genes with various origins. Therefore, more work needs to be clone for the prevention.