链脲佐菌素诱导糖尿病小鼠轮廓状乳头中味蕾特征变化

Character Changes of Taste Buds in Mice Circumvallate Papillae of Streptozotocin-Induced Diabetes

以ICR小鼠为实验对象,注射180 mg/kg剂量链脲佐菌素构建糖尿病小鼠模型,利用苏木精-伊红染色法及免疫组织化学方法分析了糖尿病小鼠味蕾形态变化和味觉信号转导相关蛋白表达变化。结果表明,对照组小鼠与糖尿病组小鼠轮廓状乳头大小及数量无显著差异;但糖尿病小鼠单个味蕾中味觉信号转导蛋白α-gustducin及磷酸脂酶Cβ2的阳性细胞数显著增加(P<0.05),而synaptobrevin-25(SNAP-25)阳性细胞显著减少(P<0.05)。综上所述,糖尿病引起的味觉变化可能来自于味信号转导蛋白表达的变化,而这些变化导致外周味觉感受信号无法有效地传导到味觉传入神经。

Diabetes mellitus （DM） are induced in ICR mice with the dose ot 180 mg/Kg ot streptozotocin（STZ） and then the morphological changes and the expression related with taste signal transduction of taste buds in vallate papillae are evaluated by hematoxylin-eosin staining and immune-histochemical methods. The results show that the number and the size of taste buds have no significant difference between control and DM mice. Whereas ,compared with control mice ,the DM mice have more number of α-gustducin and phospholipase Cβ2 positive cells per taste bud （P〈 0.05/P〈0.01）. By contrast,the DM mouse have less number of SNAP-25 cells per taste bud than that of control mice （P〈0.01）. In a word,taste changes caused by DM maybe result from the expressed change of the taste signal transduction proteins,which block the peripheral taste signal to transmit to to the gustatory afferent nerve.