动物食品中去甲基睾丸酮的纳米增强酶联免疫分析方法

A Nanoparticle-Enhanced ELISA Against Nortestosteron in Animal-Original Food

作者通过辣根过氧化物酶标记抗体（HRP-IgG）蛋白分子固定在金纳米粒子（GNP）表面的方法,制备得到一种高活性的酶-金纳米复合物（HRP-IgG-GNP）。将该HRP-IgG-GNP应用于酶联免疫分析（ELISA）方法中,针对去甲基睾酮（NT）,建立了一种高灵敏的纳米增强ELISA方法。该方法的检测灵敏度达到0.01 ng/mL,线性范围为0.03~10 ng/mL。与传统ELISA方法相比,该方法的灵敏度提高了10倍。该纳米增强ELISA方法有望开发高灵敏免疫分析方法中获得广泛应用。

Conjugating enzyme with nanoparticles is an effective approach to enhance the characteristics of enzyme molecule in biosensor. A highly active enzyme-nanoparticle complex,HRP-IgG GNP,was prepared by immobilizing horseradish peroxidase labeled antibody （HRP-IgG） onto the surface of gold nanoparticle （GNP）. The HRP-IgG GNP complex was applied to the enzyme-linked absorbent immunoassay （ELISA） and a highly sensitive nanopaticle-enhanced ELISA method was developed against nortestosterone （NT）. The sensitivity of the novel method reached 0.01 ng/mL and the linear range was 0.03-10 ng/mL, the nanopaticle-enhanced ELISA method was 10 times more sensitive than the traditional ELISA method. The above method has promising applications for developing highly sensitive immunoassay methods.