量子共振磁化干预对化学性肝损伤小鼠的保护作用

The protective effects of quantum resonance magnetic interference on chemical liver injury in mice

[目的]观察量子共振磁化干预对化学性肝损伤模型小鼠肝脏的保护作用.[方法]酒精性肝损伤模型:取雌性昆明种小鼠,随机分为正常组、模型组、直接组(1h/d)、间接组(饮用量子共振磁化水)、直-间接组(1h/d+饮用量子共振磁化水)、Vita 500组(0.01mL/g),连续干预2周.第14d除正常组外其余各组均灌胃给予560mL/L红星二锅头0.014mL/g,记录耐受时间和醉睡时间,采血,测定血清ALT,AST,γ-GT活性.四氯化碳诱导肝损伤模型:取雌性昆明种小鼠,随机分为正常组、模型组、直接组(1h/d)、间接组(饮用量子共振磁化水)、直-间接组(1h/d+饮用量子共振磁化水)、益肝灵组(70mg/kg),连续干预2周.除正常组外其余组每日腹腔注射给予1.25mL/L四氯化碳0.01mL/g.第14d处死,采血,测定血清ALT,AST,γ-GT活性.[结果]各组灌酒小鼠均醉睡,间接组和直-间接组耐受时间与模型组比较明显延长(P<0.01,P<0.05);模型组ALT活性与正常组比较明显增高(P<0.05),直接组和直-间接组ALT活性与模型组比较明显降低(P<0.05);各组睡眠时间、AST及γ-GT活性间差异均无统计学意义(P>0.05).模型组血清中ALT,AST活性明显高于正常组(P<0.01、P<0.05),γ-GT间差异无统计学意义(P>0.05);3个干预组ALT,AST,γ-GT活性与模型组比较差异均无统计学意义(P>0.05).[结论]量子共振磁化间接干预可提高对酒精的耐受性;量子共振磁化直接干预可在一定程度上预防酒精性肝损伤的发生.

OBJECTIVE To observe the protective effects of quantum resonance magnetic interference on chemical liver injury in mice. METHODS In alcoholic liver injury model and CC14 induced liver injury model, Kunming female mice were randomly divided into 6 groups, normal, model, direct intervention（1 h/d）, indirect intervention（drinking quantum resonance magnetic water）, direct and indirect intervention （1 h/d ＋ quantum magnetic resonance water drinking）, and Vita 500 （0.01 mL/g） and Yiganling （70 mg/kg） groups, respectively, and were continuously intervened for 2 weeks. Besides normal group, the other groups were daily administrated by intragastric administration with 0.014 mL/g of Red Star Erguotou with 0.01 tolerance alcohol and by mL/g of CC14 time and drun intraperitoneal injection , respectively, and the ken sleeping time were recorded in alcoholic liver injury model. All mice were executed with anesthesia at 14 days, and the activities of serum ALT, AST, and γ-GT were detected RESULTS All drunk mice were in drunken sleeping, and the tolerance time was more prolonged in indirect and direct and indirect groups than in model group（P〈0.01, P〈0.05）, and the activity of ALT was obviously increased in model group than in normal group（P〈0.05） and decreased in direct, indirect and direct groups than in model group（P〈0.05）, and the drunken sleep time, AST, γ-GT activity between the groups had no significant difference （P〈0.05）. The serum ALT and AST activity were significantly higher in model group than in normal group （P〈0.01, P〈0.05）, the ALT, AST and γ-GT activity had no significant differences in three of intervention groups than in model group （ P 〉 0.05）. CONCLUSION The quantum resonance magnetization indirect intervention can improve the tolerance to alcohol, and direct intervention can prevent the occurrence of alcoholic liver injury in a certain extent