呋喃骈呋喃木脂素的体外抗肿瘤活性

In vitro Antitumor Activities of Furan Lignans

利用 MTT快速比色法 ,以长春新碱 (Vincristin Sulfas)为阳性对照 ,研究了从玄参科(Scrophulariaceae)兰石草属兰石草 (L ancea tibetica Hook Fet Thoms)中提取的呋喃骈呋喃木脂素对人肝癌细胞 (SMMC- 772 1) ,人宫颈癌细胞 (Hela) ,中国仓鼠肺成纤维细胞 (V79)及小鼠黑色素肉瘤细胞 (B16 )的体外抗肿瘤活性 .结果显示 :呋喃骈呋喃木脂素 L C1对 4种细胞均有较强的抑制作用 ,IC50 在 4 0 .2～ 87.9μg/ m L范围 ,它对 B16的抑制作用强于长春新碱 .但是 ,L C2和 L C3均无抗癌活性 (>4 0 0 μg/ m L) ,可能 L C2和 L C3的酚羟基上的 H被糖基取代而使抗癌活性丧失 .

By using MTT calorimetric assay, the antitumor activities of furan lignans were studied,which were isolated from Lancea tibetica Hook F et Thoms on human hepatoma cell SMMC 7721,human carcinoma of uterine cervix cell Hela,hamster lung fibroblast cell V79 and mouse melanoma cell B16 in vitro .Vincristine sulfas was used as positive control, It was shown that furan lignan LC1 exhibited strong cytotoxicities on all the four tested cell lines.The IC 50 of LC1 for four cell lines was within 40.2～87.9 μg/mL, its antitumor activity to B16 cell line being stronger than that of vincristine sulfas. LC2 and LC3 had little effect on the proliferation of the tested cell lines (>400 μg/mL).It seemed that the substitution of hydrogen of phenolic hydroxyl group led to the loss of antitumor activities of LC2,LC3.