高糖培养对乳鼠海马神经元存活及c-fos蛋白表达影响

Effect of high glucose concentration on survival and c-fos expression in hippocampal neurons of neonatal rats

目的探讨高糖培养对乳鼠海马神经元存活及c-fos蛋白表达影响。方法无血清体外培养方法获得新生SD乳鼠海马神经元,分为正常组和高糖6、12、18、24h共5组。分别用PI/Hoechest33342染色法、免疫荧光细胞化学法检测检测海马神经元存活率及c-fos蛋白表达。结果高糖24h组海马神经元存活率明显低于正常组及高糖6、12、18h组(P<0.01)。高糖处理后,c-fos蛋白表达在6h开始出现,12h达高峰,此后逐渐下降(P<0.01)。结论高糖培养导致的乳鼠海马神经元存活率下降与c-fos蛋白表达有关。

Objective To investigate the effect of high glucose concentration on the survival and c-fos expression in hippocampal neurons of neonatal rats. Methods Hippocampal neurons of neonatal SD rat were cultured in serum-free meditun and divided into normal group and high glucose groups at 6, 12, 18, 24 h. The survival and c-los expression of hippocampal neurons were determined by Pl/Hoechest 33342 and immunofluorescence cytochemical stain, respectively. Results The survival rate of hippocampal neurons was significantly lower in high glucose group at 24 h than that in normal group and high glucose groups at 6, 12,18 h （P〈0.01）. After treatment with high glucose levels, c-los expression occurred at 6 h, peaked at 12 h, and deceased gradually thereafter （P〈0.01）. Conclusion The decreased survival of hippocampal neurons after treatment with high glucose levels could be related to c-fos expression in neonatal rats.