四种抗体联合检测对系统性红斑狼疮诊断的意义

Significance of combined detection of 4 kinds of antibodies in the diagnosis of systemic lupus erythematosus

目的比较分析抗双链DNA(dsDNA)、抗Sm、抗核小体、抗组蛋白抗体联合检测对系统性红斑狼疮(SLE)的诊断意义。方法选取2012年6月至2013年3月间的83例SLE患者、65例非SLE自身免疫性疾病患者(包括肌炎、硬化症、类风湿关节炎)、70例健康体检者作为对照组,使用UNION免疫分析仪全自动定量检测系统分析这四种抗体。比较四者用于SLE诊断的灵敏度、特异度以及联合检测的结果。结果抗dsDNA抗体、抗Sm抗体、抗核小体抗体、抗组蛋白抗体对SLE诊断的灵敏度分别为75%、41%、67%、79%,特异度分别为89%、100%、98%、47%。四种指标联合检测的灵敏度和特异度分别为98%和100%。结论四种抗体对SLE都具有诊断价值。四种抗体联合检测能提高SLE的检出率,尤其通过使用UNION免疫分析仪进行四项套餐式定量检测,可以为患者提供快速、可靠的检测报告,在提高医院诊疗水平的同时为患者减少了就医成本。

Objective To evaluate the significance of combined detection of anti-dsDNA,anti-Sm,anti-nuclesome,anti-histone in diagnosing systemic lupus erythematosus（SLE）. Methods 83 patients with SLE,65 patients with other autoimmune disease,inclu- ding myositis, sclerosis and rheumatoid arthritis, and 70 people as the healthy control group from June 2012 to March 2013 were re- cruited in the study. UNION automatic quantitative analyzing system was adopted to test the anti-dsDNA,anti-Sm, anti-nuclesome and anti-histone. Sensitivity and specificity were compared among the 4 indicators and the combined detection. Results The sensi- tivity of anti-dsDNA, anti-Sm, anti-nuclesome, anti-histone in the patients with SLE were 75 %, 410%, 67 %, 41% respectively, the specificity were 89 %, 100 %, 98 % and 100 % respectively. The sensitivity and specificity of the combined detection rose to 98 % and 100%. Conclusion These 4 kinds of antibodies have diagnostic value for SLE. Combined detection of the 4 kinds of antibodies can improve the positive diagnostic rate of SLE,especially by UNION immunity analyzer for quantitative detection with 4 package type which can provide fast and reliable test report for patients to improve the diagnosis of SLE while reduce the medical cost for patients.