miR-200b在结直肠癌组织中的表达情况及临床意义

Expression and clinical significance of miR-200b in colorectal cancer

目的探讨miR-200b在结直肠癌组织中的表达情况及其与患者临床病理特征的关系。方法采用实时定量PCR分析结直肠癌组织与癌旁正常组织中miR-200b的表达的差异;按ΔΔCT法对目的基因进行相对定量,结直肠癌组织中的miR-200b表达低于相应癌旁正常结直肠组织,则视为低表达,反之则为高表达;将80例结直肠癌组织按miR-200b表达情况分为高表达和低表达,分析miR-200b的表达情况与患者临床资料、病理参数之间的关系。结果结直肠组织中miR-200b表达水平明显低于癌旁正常组织(P<0.01);结直肠癌组织miR-200b的低表达率在不同性别、年龄、肿瘤部位患者间的差异均无统计学意义(P>0.05);miR-200b的表达水平降低与组织学分级、TNM分期、血清CEA水平密切相关。结论 miR-200b很可能会成为结直肠癌早期诊断及预后判断的指标。

Objective To study the expression of miR-200b in colorectal cancer and its correlation with clinical features and pathological characters.Methods To detect the expression of miR-200b in colon cancer tissues and corresponding normal tissues by qPCR,and relatively quantify the level of target gene by ΔΔCT method.The expression level of miR-200b in colorectal cancer tissue is lower than corresponding adjacent normal colorectal tissue,which is regarded as a low expression,otherwise is regarded as high expression.80 colorectal cancer tissue samples were judged as high expression or low expression,then the comparison of miR-200b expresson were did among patients with different clinical features and pathological parameters.Results miR-200b expression level in the colorectal cancer tissue was significantly lower than the corresponding adjacent normal colorectal tissue（P＆lt;0.01）.Decreased miR-200b expression rates of colorectal cancer tissue showed no significant difference in different clinical features,such as sex,age and tumor site（P＆gt;0.05）.However,the decreased expression rates of miR-200b were obviously related with pathological grade of colorectal cancer,TNM stages and serum CEA level.Conclusion miR-200b may become a new tumor marker in the early diagnosis and prognosis of colorectal cancer.