VEGF、HIF-1α、CEA测定对胸腔积液和腹水良恶性鉴别的意义

Diagnostic value of VEGF,HIF-1α and CEA detection in the identification of benign or malignant pleural effusions and ascites

目的探讨血清血管内皮生长因子(VEGF)、低氧诱导因子(HIF)-1α、癌胚抗原(CEA)检测在鉴别良、恶性胸腔积液和腹水中的价值。方法采用酶联免疫吸附试验(ELISA)与化学发光免疫分析(CLIA)方法对200例良、恶性肿瘤患者胸腔积液和腹水中的VEGF、HIF-1α、CEA进行了检测。结果 VEGF、HIF-1α、CEA水平在良、恶性胸腔积液和腹水间均存在明显差异(P<0.01),三者在恶性胸腔积液和腹水检测中的阳性率分别为71.2%、56.6%、62.8%,联合测定后阳性率提高到80.5%。结论 VEGF、HIF-1α、CEA测定对于良、恶性胸腔积液和腹水性质的鉴别具有重要价值,VEGF、HIF-1α、CEA联合检测可提高诊断率。

Objective To investigate the diagnostic value of vascular endothelial growth factor（VEGF）,hypoxia-inducible factor-1α（HIF-1α） and carcino-embryonic antigen（CEA） in the identification of benign or malignant pleural effusions and ascites.Methods enzyme-linked immunoadsordent assay（ELISA） and chemiluminescence immunoassay（CLIA） were performed on pleural effusions and ascites from 200 patients with benign or malignant tumors to detect VEGF,HIF-1α and CEA.Results There is Significant difference between benign and malignant pleural effusion and ascites in VEGF、HIF-1α and CEA levels（P＆lt;0.01）.The positive detection rates of VEGF,HIF-1α and CEA detection is 71.2%,56.6% and 62.8%,respectively in malignant pleural effusing and ascites,while using combined test the positive detection rate increased to 80.5%.Conclusion VEGF,HIF-1a and CEA detections are valuable for diagnosing malignant pleural effusions and ascites,and the positive diagnostic rate can be improved significantly by using combined VEGF,HIF-1a and CEA tests.