牡丹CDDP分子标记引物筛选及多态性分析

Screening of CDDP Molecular Marker Primers for Tree Peony and Analysis of Its Polymorphism

验证了牡丹CDDP反应体系的稳定性和重复性。在21条CDDP引物中筛选出了20条多态性引物,其中18条对牡丹品种具有较强的鉴别能力。用20条CDDP多态性引物对22个国内外牡丹品种进行PCR扩增,每条引物的扩增条带从3～25条不等,共获得297条DNA片段,其中多态性片段276条,多态性比率为92.93%,平均每条引物扩增出14.85种多态性基因型。多态性好的18条引物对22份牡丹材料的位点多态性信息量分布在0.8379～0.9597之间,平均达0.9124。可以运用CDDP分子标记技术对牡丹种质资源进行遗传多样性分析、品种鉴定、遗传图谱构建、基因定位等研究。

The stability and repeatability of conserved DNA-derived polymorphism（CDDP） reaction system for tree peony were tested and verified.Twenty out of twenty-one CDDP primes were polymorphic for tree peony genome,and 18 primers had stronger ability to discriminate tree peony cultivars.The applicability of 20 CDDP primers to 22 tree peony cultivars from domestic and abroad was analyzed in the present paper.The results showed that each primer could amplify 3~25 bands and 276 polymorphic fragments were detected among the total 297 fragments amplified.The polymorphism rate was 92.93% with a mean of 14.85 polymorphic bands for each primer.The polymorphism information content（PIC） of the 18 primers ranged from 0.8379 to 0.9597 with an average of 0.9124.Thus,CDDP molecular marker technology can be used to analyze the genetic diversity,identification of breeds,construction of genetic maps,gene mapping researches for tree peony germplasm researches.