核盘菌和灰葡萄孢菌CCCH锌指蛋白基因的鉴定和比较分析

Identification and Comparative Analysis of CCCH Zinc Finger Genes from Sclerotinia sclerotiorum and Botrytis cinerea

CCCH锌指蛋白(zinc finger proteins)广泛分布于动物、植物和微生物之中,在RNA代谢过程中起着重要作用,通过鉴定核盘菌和灰葡萄孢菌Zf基因,为基因克隆、表达分析和功能研究奠定基础。本研究以核盘菌和灰葡萄孢菌全基因组序列为材料,运用生物信息学的手段开展Zf基因的全基因组鉴定和分析。经分析,核盘菌和灰葡萄孢菌各有11个Zf基因,它们的开放性阅读框全长分别为633～3741bp和633～3486bp;共检测到55个CCCH锌指基序,以C-X7-C-X5-C-X3-H(22个)和C-X8-C-X5-C-X3-H(21个)最为常见,其中C-X8-C-X6-C-X3-H和C-X7-C-X6-C-X3-H为2种新发现的基序类型;进化分析结果表明,22个Zf基因聚为4组,除SS1G_13631、SS1G_09360、BC1T_00310和BC1T_05217外,核盘菌和灰葡萄孢菌的Zf基因分别位于不同的小分支,该结果与Zf基因的基因结构、基序数量和分布情况一致。

CCCH zinc finger proteins（Zf） are widely distributed in animals,plants as well as microorganisms,and play important roles in RNA metabolism.Identification of Zf genes in Sclerotinia sclerotiorum and Botrytis cinerea will provide a basis for gene cloning,expression as well as function analysis.Genome-wide survey and comparative analysis of Zf genes were performed in S.sclerotiorum and B.cinerea by bioinformatics methods.Eleven Zf gene members were identified from S.sclerotiorum and B.cinerea,respectively.Full-length of open reading frames were of 633-3741bp in S.sclerotiorum and 633-3486bp in B.cinerea,respectively.Totally 55 CCCH zinc finger motifs were identified.Besides the two most abundant CCCH zinc finger motifs,C-X7-C-X5-C-X3-H（22） and C-X8-C-X5-C-X3-H（21）,C-X8-C-X6-C-X3-H and C-X7-C-X6-C-X3-H were found to be two novel CCCH zinc finger motifs.Phylogenetic analysis results indicated the 22 CCCH zinc finger proteins were clustered into 4 groups.Except for SS1G_13631,SS1G_09360,BC1T_00310 and BC1T_05217,other Zf genes from S.sclerotiorum and B.cinerea belonged to different subfamilies in the form of orthologous pairs,and the results were consistent well with gene structures,motif numbers as well as distributions.