有机磷农药降解菌ZHJ6的γ辐照诱变

The Mutant Strain of ZHJ6 Degrading Organophosphorous Pesticide by^(60) Co-γ Irradiation

为获取对甲胺磷农药降解率更高的菌株,利用60Co-γ射线对能够降解甲胺磷农药的草酸青霉ZHJ6进行辐照诱变研究。结果表明:草酸青霉ZHJ6对60Co-γ射线比较敏感,容易被辐照灭活,当吸收剂量继续增大,达到2.1kGy时,存活率变为0.04%左右。辐照后的菌株经过初筛和复筛,筛选出A17和A18两株菌株。筛选出的菌株比出发菌株A0的对甲胺磷的降解率要高出10%以上,而对氧化乐果、辛硫磷和草甘磷的降解能力也增强。经过连续5代传代培养,诱变菌株在第6d对甲胺磷农药的变异系数为1.2%,遗传特性稳定性好。

The strain of Penicillium oxalicum ZHJ6 that can degrade methamidophos was employed to obtain the mutant stain which has higher degradation rate than original strain by60Co-γ irradiation.Results showed that the Penicillium oxalicum ZHJ6 was sensitive to60Co-γ irradiation,and was easy to be killed by60Co-γ irradiation.Under the absorbed dose of 2.1kGy,the survival rate of the strain was 0.04%.Two strains of A17 and A18 were obtained from the irradiated strains after first-and second-screening and the degradation rate of methamidophos of A17 and A18 strains were 10% higher than that of A0 strain（original stain）.Moreover,the abilities to degrade folimat,phoxim and glyphosate were improved.Through 5 generations,the variation coefficient in degradation rate of methamidophos in the 6th day was 1.2%,showing that the new strains had hereditary stability.