珍珠黄杨愈伤组织诱导的研究

Study on Callus Induction of Buxus sinica var. parvifolia

[目地]探讨珍珠黄杨愈伤组织的诱导条件.[方法]以MS为基本培养基,采用2.0、1.6、1.2和0.8 mm4种不同直径茎段为外植体,采用NAA和6-BA 2种激素不同浓度组合诱导愈伤组织;采用2,4-D、NAA和6-BA 3种激素的不同浓度组合对珍珠黄杨愈伤组织进行继代培养.[结果]使用珍珠黄杨茎段为材料时,最佳愈伤组织诱导激素组合为1.0 mg/L NAA ＋2.0 mg/L 6-BA,出愈率为69.84％;最佳取材直径为1.2～1.6mm;最佳愈伤继代激素组合为2.0 mg/L2,4-D＋ 1.0 mg/L 6-BA.[结论]该研究为珍珠黄杨的高速繁殖研究奠定了基础.

[Objective]To discuss the inducing of callus of Buxus sinica var.parvifolia.[Method]Taking MS as basic medium,supplemented with NAA and 6-BA at different concentration,the stem with various diameters(2.0,1.6,1.2,0.8 mm) of Buxus sinica var.parvifolia was used to induce callus.The callus was subcultured on MS medium adding 2,4-D,NAA and 6-BA at different concentration.[Result] Taking stem as experimental material,the optimal callus induction medium was 1.0 mg / L NAA + 2.0 mg / L 6-BA and the inductive rate was 69.84%.The best stem diameter was 1.2-1.6 mm.The optimal subculture medium was 2.0 mg / L 2,4-D + 1.0 mg / L 6-BA.[Conclusion]This experiment will lay the foundation for research on the rapid propagation of Buxus sinica var.parvifolia.