No051内生真菌次生代谢产物量与培养时间的关系研究

Study on Relationship between the Amount of Metabolites of No051 Endophytic Fungi and Incubation Time

[目的]研究No051内生真菌次生代谢产物量与培养时间的关系。[方法]以从辽宁省西北地区根系土壤中分离得到的No051内生真菌为材料,对其进行液体培养,分别在培养5、9、13、17、21和25 d后进行次生代谢产物干物质量的测定,同时进行高效液相色谱分析。[结果]液体培养9 d为次生代谢产物产生的高峰期;高效液相色谱分析表明该时期产生的次生代谢产物中包含的活性物质较多。[结论]该研究为No051内生真菌次生代谢产物的进一步研究提供了理论依据。

Abstract [ Objective] The paper was to study the relationship between the amount of metabolites of No051 endophytic fungi and incubation time. [ Method ] The No051 endophytic fungi isolated from root- zone soil in northwest area of Liaoning province was used as materials, and it was cultured in liquid. The dry matter weight of secondary metabolites was measured after 5, 9, 13, 17, 21 and 25 days, respectively, and high performance liquid chromatography （HPLC） analysis was carried out. [ Result] The production of secondary metabolites reached the peak in 9 days - cultivation. The HPLC analysis showed that higher content of active substances in the secondary metabolites in 9 days as well. [ Conclu- sion] The study provided a theoretical basis for the further study of secondary metabolites of No051endoohvtic fungi.