多杀性巴氏杆菌生物被膜扫描电镜研究

Scanning Electron Microscopic Observation on the Biofilms of Pasteurella multocida

[目的]利用扫描电镜研究多杀性巴氏杆菌生物被膜的独特结构。[方法]在扫描电镜生物样品制备常规方法和改进方法的基础上,对从广东病鸡分离出的3株多杀性巴氏杆菌的表面形貌进行比较。[结果]改进方法较常规方法更明显地获得了细菌生物被膜三维立体结构观察的效果。[结论]扫描电镜可观测到多杀性巴氏杆菌生物被膜直观的三维构象,为细菌生物被膜的进一步研究提供了直观依据。

Abstract [ Objective ] Scanning electron microscope （SEM） was used to observe the unique structure of the biofilm of Pasteurella muhocida. [ Method] Three strains of Pasteurella muhocida isolated from diseased chickens of Guangdong were treated with conventional method and im- proved method of biological sample preparation for SEM. Then their surface morphology was detected by SEM. [ Result] Using the improved method is more obvious to observe the three-dimensional structures of bacterial biofilm than using the conventional method. [ Conclution ] SEM can detect the three-dimensional structures of bacterial biofilm. It provides an intuitive basis for further studies of Pasteurella muhocida＇ s hie- film.