STAT3与SOCS3在子宫内膜腺癌中的表达及生物学行为相关性分析

Biological behavior analysis and expression of STAT3 and SOCS3 in endometrial carcinoma by using tissue chip technique

目的探讨STAT3、SOCS3基因蛋白在子宫内膜腺癌中的表达及意义,以期了解这两种蛋白在子宫内膜腺癌癌变过程中的作用。方法收集46例手术切除的子宫内膜腺癌组织标本,同时取30例正常子宫内膜组织作为对照,运用组织芯片技术以及免疫组织化学的方法检测子宫内膜癌组织、正常子宫内膜组织中STAT3、SOCS3蛋白的表达。结果 46例子宫内膜癌组织中STAT3的阳性表达率为76.1%,SOCS3的阳性表达率为19.6%,两者在子宫内膜腺癌与正常子宫内膜组织之间的阳性率表达差异有显著的统计学意义(P<0.05)。二者的阳性表达与子宫内膜腺癌患者的肿瘤分化程度有关,与手术病理分期、肌层浸润及绝经情况等临床特征无相关性(P>0.05)。子宫内膜腺癌中STAT3与SOCS3呈负相关。结论子宫内膜腺癌中STAT3呈高表达,SOCS3呈低表达,提示STAT3促进了子宫内膜腺癌的发生发展,而SOCS3抑制子宫内膜腺癌癌变的作用。

Objective To investigate the expression of STAT3 and SOCS3 protein in endometrial cancer of uterine and its clinicopathologic significance in order to detect if these protein have effect on endometrial carcinoma. Methods Tissue chip technique and immunohistochemical staining method were used to examine the expression of STAT3 and SOCS3 in 46 cases of endometrial carcinoma and in 30 cases of the normal endometrium. The relationship of these subjects was analyzed with spearman rank correlation. Results The positive rates of STAT3 and SOCS3 in endometrial carcinoma were demonstrated significant difference compared with normal endometrial tissue. The expression of STAT3 and SOCS3 had relationship with histological grade in endometrial carcinoma. The differences reached significant value （ P 〈 0.05 ）. Negative correlation was found between the expression of STAT3 and SOCS3 in endometrial carcinoma （ r = - 0. 494, P = 0. 004 〈 0.05 ）. Conclusion The over - ex- pressions of STAT3 and the down - regulated expression of SOCS3 in endometrial carcinoma may play an important role in the carcinogenesis and progression of endometrial carcinoma.