Ouabain诱导沙鼠蜗外电诱发听性脑干反应变化研究

The study for the Gerbil electrically auditory brainstem responses change induced by Ouabain

目的沙鼠耳蜗圆窗应用毒毛旋花甙G(Ouabain),观察沙鼠蜗外电诱发听性脑干反应(EABR)的变化。方法显微镜下将浸有50μl Ouabain(1 mM)的明胶海绵置于沙鼠耳蜗圆窗膜表面,观察应用Ouabain 24 h、96 h后听觉脑干反应(ABR)、耳蜗微音电位(CM)、蜗外电诱发听性脑干反应(EABR)阈值变化。结果沙鼠圆窗应用Ouabain 24h、96 h后ABR、EABR阈值明显升高,差异具有统计学意义(P<0.05),CM阈值无明显变化,差异无统计学意义(P>0.05)。结论沙鼠圆窗膜表面应用50μl Ouabain(1 mM)24 h、96 h后可导致沙鼠ABR、EABR阈值明显升高,而CM阈值无明显变化。

Objective To observe the Gerbil electrically audiotory brainstem responses change after Ouabain was applied to the round window of the gerbil. Methods A piece of gelfoam immerged with Ouabain solution （50 μl mM） was used to cover on the round window membrane of the cochlea. After applying ouabain in the cochlea for 24 h and 96 h, the threshold changes of audiotory brainstem responses （ ABR）, Cochlear microphanics （ CM）, electrically audiotory brainstem responses （EABR） were observed. Results Ouabain application for 24 h and 96 h induced the threshold of ABR and EABR to increase obviously. The difference had statistical significance（ P 〈0.05）. But the threshold of CM remained no obvious change, the difference had no statistical difference （ P 〉 0.05 ）. Conclusion Ouabain application on the round window membrane of the gerbil cochlea for 24 h and 96h may make the threshold of ABR and EABR increase, but the threshold for CM had no obvious change.