摘要
目的:观察大鼠腰部背根神经节经接受脉冲射频后的急性期改变。方法:选择Wistar大鼠30只,随机分为观察组和对照组各15只,两组大鼠均经完全麻醉后暴露椎旁肌肉。观察组给予左侧L4背根神经节接受脉冲射频处理(2Hz,30s),对照组无脉冲射频。1h后分离所有大鼠的双侧L3、L4、L5背根神经节,固定染色后分别行光镜及电镜观察细胞形态学改变。结果:对照组神经节细胞及细胞内超微结构未见明显异常,神经纤维同样呈现正常形态。观察组光镜下可见轻微的组织水肿,电子显微镜下发现部分神经节细胞出现内质网增大、线粒体破坏、细胞膜及核膜的完整性消失及有髓神经纤维髓磷脂脱落。观察组计数到的中位细胞异常率为78.3%,对照组为24.5%(P<0.01)。结论:脉冲射频在急性期可造成神经节细胞的损害,病理改变以细胞器破坏及有髓神经纤维髓磷脂脱落为主。
Objective:To observe the acute phase changes in the rat lumbar dorsal root ganglion after pulsed radiofrequency(PRF)treatment.Methods:30 rats were divided into two groups randomly(the observation group and the control group with 15 rats per group),and the paravertebral muscles were explored after deeply anesthesia.The left L4 dorsal root ganglion of the observation group was given PRF processing(2Hz 30s);no PRF for the control group.The left L3,L4,L5 dorsal root ganglion of all the rats were separated 1hour later;then fixed and stained.Morphological changes were observed by light microscopy and electron microscopy.Results:For the control group,ganglion cells and ultrastructure of cells were not abnormal and the nerve fibers were in the normal form.For the observation group,light microscopy showed slight edema and electron microscopy showed the increasing of endoplasmic reticulum,mitochondrial damage,disappearing of the integrity of the cell membrane and nuclear membrane in part of the ganglion cells,and delamination of myelin of myelinated nerve fibers.The median cell abnormality rate of the observation group and the control group was 78.3% and 24.5% respectively(P<0.01).Conclusion:Pulsed radiofrequency can cause ganglion cell damage in the acute phase and the main pathological changes are damage of organelles and delaminated myelin.
