桂皮醛诱导人胃癌BGC-823细胞凋亡及相关分子机制的探讨

Discussion on Cell Apoptosis and Its Related Molecular Mechanisms of Human Gastric Carcinoma Cell Line BGC-823 Induced by Cinnamaldehyde

目的:探讨桂皮醛对人胃癌BGC-823细胞增殖、凋亡及其相关分子机制。方法:采用MTT法检测桂皮醛对人胃癌BGC-823细胞增殖的影响;采用流式细胞仪检测桂皮醛诱导的凋亡,Hoechst 33342染色法观察凋亡形态的变化;RT-PCR法检测桂皮醛对人胃癌BGC-823细胞相关凋亡基因的影响;Western Bolt方法检测桂皮醛对人胃癌BGC-823细胞相关凋亡蛋白的影响。结果:与对照组相比,桂皮醛有抑制人胃癌BGC-823细胞增殖的作用(P<0.01);桂皮醛能诱导凋亡的产生;桂皮醛能下调凋亡相关基因Bcl-2、Bcl-xL、Survivin,上调凋亡相关基因Bax、Bak;桂皮醛能下调凋亡相关蛋白Bcl-2、Procaspase-3,上调凋亡相关蛋白Bax。结论:桂皮醛对人胃癌细胞BGC-823的增殖具有抑制作用并能诱导凋亡,可能与内源性凋亡途径的激活有关。

This article was aimed to investigate the cell proliferation, cell apoptosis and its related molecular mechanisms of the human gastric carcinoma cell line BGC-823 in vitro after treatment with cinnamaldehyde. The MTT Assay demonstrated the inhibitory effect of cinnamaldehyde. And the Flow Cytometry was used to determine its induction of cell apoptosis. The Hoeehst 33342 was used to observe morphological changes during apoptosis. Moreover, quantitative real time PCR and western blot analysis were used to detect the effect of cinnamaldehyde on human gastric carcinoma cell line BGC-823. The results showed that compared with the control group, einnamaldehyde had inhibitory effect on human gastric carcinoma cell line BGC-823 （P 〈 0.01）. It showed that cinnamaldehyde induced apoptosis through the downregulation of Bcl-2, Bcl-xL and Survivin expression, upregulation of Bax and Bak expression, downregulation of Bcl-2 and Procaspase-3, and upregulation of BAX. It was concluded that cinnamaldehyde had inhibitory effect on the proliferation of human gastric carcinoma cell line BGC-823 and induced apoptosis. It may be related to the activation of the endogenous apoptosis pathway.