基于RLCKVⅡ基因序列的蜘蛛抱蛋属系统发育分析

Phylogenetic Analysis of Aspidistra Inferred from Sequences of RLCKVⅡ Gene

蜘蛛抱蛋属(Aspidistra Ker-Gawl.)是单子叶植物中同一属内物种多样性最为丰富的类群之一.由于缺乏分子序列方面的研究资料,该属种间系统发育关系一直都不清楚.本研究对20种具有代表性的蜘蛛抱蛋属植物的RLCKVⅡ基因进行了测序分析.结果表明,采用分子系统学证据所构建的该属系统发育关系不支持先前基于形态证据所建立的分类系统;蜘蛛抱蛋属植物复杂的平行进化关系很可能是该属分化早期频繁种间杂交的结果.RLCKVⅡ基因适用于禾本科、棕榈科和天门冬科等类群,可作为研究单子叶植物系统发育关系的备选基因.

Aspidistra Ker-Gawl. （Asparagaceae, subfamily Nolinoideae）, with high species diversity, is one of the biggest genera in monocotyledon. However, phylogenetic relationships among species in Aspidistra remain unknown, due to little molecular sequence analysis. In this study, molecular phylogeny of Aspidistra was conducted based on the sequence variations of nuclear DNA RLCKVII for 20 representative species of Aspidistra. The results showed that the molecular phylogenetic relationships among Aspidistra species were not consistent well with the infrageneric classification based on the morphological characters. The complexity of parallel phylogenetic relationships indicates that Aspidistra might have experienced frequent episodes of interspecific hybridization at its initial stages of diversification. The RLCKVII gene can be used for species identification in the families Poaceae, Palmae and Asparagaceae, suggesting that RLCKVII is a useful candidate gene for phylogenetic analysis of monocotyledon.