复配絮凝剂用于小儿咳喘灵原药水提液絮凝效果研究

Study on flocculation effect of water-extraction solution of Xiao'er Kechuanling Oral Liquid with composite flocculants

目的 优化小儿咳喘灵口服液的絮凝除杂工艺，保留有效成分，提高药液澄清度。方法 针对小儿咳喘灵原药水提液的杂质成分，采用单宁和壳聚糖复配的方法，即在第1和第2阶段快速搅拌开始时分别加入单宁溶液和壳聚糖溶液，并以绿原酸保留率、蛋白质和鞣质去除率以及处理后上清液的浊度为指标，分别考察了絮凝剂配比及用量、絮凝温度、搅拌桨叶端线速度和搅拌时间对絮凝效果的影响。结果 通过实验得到最佳絮凝工艺条件：单宁和壳聚糖的用量分别为0.250、0.500 g/L，絮凝温度为20～30 ℃，快搅速度和快搅时间分别为0.262 m/s和1.0 min，第1阶段的慢搅速度和慢搅时间分别为0.131 m/s和2.0 min，第2阶段的慢搅速度和慢搅时间分别为0.105 m/s和5.0 min。该条件下绿原酸的保留率为92.6%，蛋白质和鞣质的去除率分别为55.8%、70.0%，处理后药液放置24 h后浊度为4.4 NTU，放置90 d后浊度为4.7 NTU。结论 实验表明单宁和壳聚糖复配用于小儿咳喘灵原药提取液能有效提高杂质去除率，同时提高药液的澄清度和稳定性。

Objective To optimize the purifying process of water-extraction solution of Xiao’er Kechuanling Oral Liquid （XKOL）, reserve more active components, and improve the clarity. Methods Given the impurity composition of the water extraction solution, the flocculation effect of tannin and chitosan compositely used was analyzed in this paper. Tannin was added in the water extraction solution in the first phase at the beginning of fast mixing, while chitosan was added in the second phase. Supernatant turbidity, the retention rate of chlorogenic acid and the removal rate of impurity were set as main indexes to estimate the influences of the dosage of the two flocculants, temperature, mixing speed, and time on flocculation. Results The optimal flocculating process conditions were as follows： the dosage of tannin and chitosan were 0.250 and 0.500 g/L, the flocculation temperature was 20-30 ℃, the fast mixing speed and time were 0.262 m/s and 1.0 min, the slow mixing speed and time in the first phase were 0.131 m/s and 2.0 min, while in the second phase were 0.105 m/s and 5.0 min. Under the above conditions, the retention rate of chlorogenic acid was 92.6%, the removal rates of tannin and protein were 55.8% and 70.0%, the supernatant turbidity was 4.4 NTU after 24 h and 4.7 NTU after 90 d. Conclusion The experimental results turn out that the method of using the composite flocculants in the treatment of water extraction solution of XKOL could improve the removal rate of impurity, liquid clarity, and stability.