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人4IgB7-H3对结肠癌细胞SW480中β3GnT8表达的影响

Impact of 4IgB7-H3 on β3GnT8 Expression in Human Colon Cancer Cell Line SW480
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摘要 目的探索人结肠癌细胞SW480转染4IgB7-H3后对糖基转移酶133GnT8和细胞表面多聚乳糖胺糖链表达的影响。方法脂质体介导的重组表达载体plRES2-EGFP／4IgB7-H3转染SW480细胞，筛选并获得稳定表达4IgB7-H3的亚克隆细胞株；RT-PCR、WesternBlot检测B3GnT8的表达；流式细胞术检测多聚乳糖胺寡糖链的表达。结果成功获得稳定表达4IgB7-H3的亚克隆细胞株SW480／4IgB7-H3；转染4IgB7-H3的SW480细胞中B3GnT8和多聚乳糖胺糖链的表达均明显下调（P〈0．05）。结论人肠癌细胞中4IgB7-H3过表达能抑制p3GnT8的表达，进而抑制多聚乳糖胺糖链的表达。 Objective To explore the expressions of glycosyltransferase ]3 3GnT8 and polylactosamine on cell surface in human colon cancer cell line SW480 transfected with Flow cytometry detcted lactosamine oligosaccharide chain expression.Results 4IgB7-H3 recombinant expression vector was successfully constructed.Then stably transfected SW480 cell line was got.Both the expressions of [3 3GnT8 and poly lactose amine sugar chain decreased significantly（P 〈 0.05） after transfection.Conelusi0a 4IgB7-H3 overexpression in human colorectal cancer cells can inhibit [3 3GnT8 production,and thereby inhibit poly galoctosamine extension.
作者 石小蕊 姜彧滕 邹士涛 倪建龙 高利平 徐徐 于美云 姜智 周迎会 吴士良
机构地区 苏州大学医学部生物化学与分子生物系暨生化工程研究所 苏州大学 无锡市第四人民医院
出处 《中国血液流变学杂志》 CAS 2013年第2期207-210,225,共5页 Chinese Journal of Hemorheology
基金 国家自然科学基金资助项目（31170772,30901313）,苏州市科技项目（SYS201208）
关键词 4IgB7-H3 转染 SW480 B 3GnT8 多聚乳糖胺 4IgB7-H3 transfection SW480 13 3GnT8 polylactosamine
分类号 R735.35 [医药卫生—肿瘤]
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参考文献10

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中国血液流变学杂志
2013年 第2期

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