无创检测肝储备功能的脉搏色素密度法

Pulse dye densitometry method for non-invasive measurement of hepatic functional reserves

针对当前肝脏储备功能检查存在需分时采集动脉血进行分光光度分析、依赖手工操作造成测量精度低和测量时间长,以及医务人员易被感染等问题,通过建立和分析吲哚氰绿色素的稀释及排泄特性曲线,研究一种无创、连续检测肝储备功能参数的脉搏分光光度测量方法。在静脉注入吲哚氰绿指示剂后,利用940nm和805nm的双波长光源同步采集脉搏波信号,根据血液中色素浓度的时间变化率,计算得到血浆消失率和15min滞留率等肝储备功能参数。经与采血的分光光度法对比,该检测方法的平均相对误差小于5%,可以为临床的肝切除手术提供准确的肝储备功能评价结果。

The exist several problems in current measurement of hepatic functional reserves, such as multiple blood samplings for spectrophotometry, lower precision and longer time taken by manual operation, and possible infection risk. In order to solve these problems, a noninvasive and continuous method based on pulse sepctrophotometry for detection of hepatic functional reserves is presented by setting up and analyzing Indocyanine Green （ICG） dye dilution and clearance curves. While ICG is injected intravenously, two light sources at 940 nm and 805 nm wavelengths are used to implement data acquisition by Photoplethysmography （PPG）. The ICG disappearance rate in blood plasma （K value） and 15 rain retention ratio （R15 value） as hepatic functional reserves parameters can be determined by calculating time-varying ICG concentration values. Compared with blood sampling method, the mean relative error of this new method is below 5 %. The results confirm its usefulness in evaluating hepatic functional reserves for clinical liver removal surgery.