高良姜总黄酮对铅中毒致小鼠脑、肾氧化应激的保护作用研究

Protective Effect of Total Flavonoids from Alpinia officinarum Hance on Brain and Kidney Oxidative Stress in Mice Exposed to Lead Acetate

[目的]探讨高良姜总黄酮(Total flavonoids from Alpinia officinarum Hance,TFAO)对铅中毒小鼠脑、肾脏氧化应激的保护作用。[方法]60只小鼠分为正常组,模型组,阳性组,TFAO低、中、高剂量组。通过腹腔注射醋酸铅建立铅中毒模型,灌胃给予受试物。实验结束后,测定小鼠体重、脏器指数,血液及组织铅浓度,脑、肾氧化应激参数,如还原型谷胱甘肽(glutathione,GSH)、丙二醛(malondialdehyde,MDA)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)、超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)等指标。[结果]高浓度TFAO对小鼠血液及组织中铅含量有明显降低作用,且未造成其他必需元素如锌的流失。与模型组相比较,TFAO可显著增加小鼠脑、肾GSH含量和抗氧化酶活性,并能显著降低MDA水平。[结论]TFAO可通过提高铅中毒小鼠组织中抗氧化酶的活性,改善脂质过氧化,从而发挥其对铅诱导的脑、肾氧化应激的保护效应。

[Objective] To investigate the protective effect of total flavonoids from Alpinia offtcinarum Hance（TFAO） on lead acetate induced oxidative stress in brain and kidney of mice. [Methods] All 60 mice were randomly divided into 6 groups： normal control group, lead group, positive control group, lead＋TFAO（I00, 300, 500 mg＇kg-1） groups. Mice except normal control group received intraperitoneal injections of lead acetate every other day, and dif- ferent test substances were administrated to mice orally once a day. After the last dose was administrated, the body weight, viscera index, lead and zinc con- tents in blood, brain and kidney, oxidative stress parameters such as glutathione（GSH）, glutathione peroxidase（GSH-Px）, superoxide dismutase（SOD）, cata- lase（CAT） and malondialdehyde （MDA） in brain and kidney were determined. [Results] TFAO in high dose （500 mg＇kg-1 could lower the lead level in blood, brain, and kidney significantly. The other trace elements such as zinc had no significant decrease after TFAO treatment in blood, however, TFAO in high doses could increase the zinc content in brain, and kidney sigrfificantly. Three different doses of TFAO（100, 300, 500 mg＇kg-1） could increase the activity of antioxidant enzymes and the content of GSH, decrease the content of MDA in brain and kidney significantly. [Conclusion] TFAO might im- prove antioxidant defense system, reverse hpid peroxidation and protect brain and kidney against lead induced oxidative stress in mice significandy.