不同浓度艾烟长期干预对大鼠血清vWF及ox-LDL表达的影响

Effect of Long-term Intervention of Moxa Smoke with Different Concentrations on vWF、ox-LDL in Rats Blood Serum

目的:观察不同浓度艾烟对大鼠血液微循环的影响,为临床安全使用艾烟提供参考。方法:将168只wistar大鼠随机分为四组,每组42只分别暴露于0%、10%、40%、70%浓度(以染毒柜不透光率度量),每天20min,26周后取材,ELISA法检测不同浓度组大鼠血清中vWF、ox-LDL的含量。结果:vWF含量随艾烟浓度升高而逐渐下降,低、中、高浓度组vWF含量与空白对照组相比均有统计学意义;中浓度组艾烟干预后,血清中ox-LDL含量显著性升高,其余各组ox-LDL含量与空白组相比无统计学意义。结论:1)艾烟可使血小板聚集程度下降,提示艾烟可能具有改善微循环,从而促进机体新陈代谢的作用。2)低浓度艾烟对血管内皮未见明显损伤。

Objective：To observe the effect of moxa somke with different concentrations on blood microcirculation and provide reference on security of moxa smoke in clinic.Methods：A total of 168 rats were randomly divided into 4 groups,42 in each group.Rats in control group,low concentration goup,medium concentration group,high concentration group were respectively exposed to moxa smoke of 0%,10%,40%,70% concentration （in terms of opacity） for 20 min each day,after 26 weeks rats were executed and drawn materials and with ELISA method ox-LDL、vWF in rats blood serum of different group were tested.Results：vWF showed downtrend as the concentration of moxa smoke ascended,which of low,medium,high concenteation group significantly declined compared with control group;It has significantly rise in medium cencentrtion group compared with control group,the rest groups had no significant change by contrast with control group.Conclusion：1） The rise of concentration of moxa smoke brings down Platelet aggregation,promotes body metabolism and improves microcirculation.2） Low concentration of moxa smoke indicates no injury on vascular endothelial.