转染突变的BMPsII型受体使NIH3T3细胞的增殖活性降低

Negative effect on the proliferation of NIH3T3 fibroblasts by gene transfection of a truncated type II receptor for BMPs Ⅱ

目的 :探讨转染BMPsII型突变受体对NIH3T3细胞生物学行为的影响。方法 :用携带BMPsII型突变受体的cDNA的真核表达载体转染NIH3T3细胞 ,对NIH3T3细胞进行生长曲线、MTT比色、流式细胞仪分析、BrdU检测。结果 :转染细胞的增殖活性和DNA合成下降。结论

AIM: To examine the effect of gene transfection of a truncated type Ⅱ receptor for BMPs Ⅱ on NIH3T3 cells. METHODS:The cDNA for a truncated BMP-2 type II receptor was transfected into NIH3T3 cells, and the biological behaviors of NIH3T3 cells were investigted. RESULTS: The transfected cells showed decreased proliferation ability and DNA synthesis. CONCLUSION:The gene transfection has a negative effect on the proliferation of NIH3T3 cells and DNA synthesis. [