E-Cadherin在修复性牙本质形成中的免疫组化研究

Immunohistochemistry study of E-cadherin in reparative dentinogenesis

目的 :观察细胞粘附分子E -cadherin在修复性牙本质形成中的免疫反应和免疫定位。方法 :在大鼠上、下颌第一磨牙近中面制备单面洞 ,分别观察 3、15和 30d。标本行常规组织学处理。利用鼠抗E -cadherin和免疫组化SABC法 ,对标本进行标记 ,HE复染。结果 :E -cadherin在术后 3d成牙本质细胞染色较弱 ,牙髓细胞为阳性 ;15d后 ,成牙本质细胞样细胞和牙髓细胞染色阳性 ;30d后 ,成牙本质细胞样细胞出现极化 ,胞浆阳性染色明显。结论 :E

AIM: To investigate the immunological reactivity of E-cadherin in the reparative dentinogenesis. METHODS: The cavity was prepared on the mesial surface of the first molar of Wistar rats. After the periods of 3, 15, 30 days of post-operation, animal were sacrificed and paraffin sections were prepared through histological process. SABC method was used to detect the reactivity using rat anti-E-cadherin antibody. RESULTS: The reactivity was not evident in odontoblasts of 3 days group, but was evident in dental pulp cells; the positive staining was observed in odontoblast-like cells and pulp cells after 15 days; in 30 days, the polarized odontoblast-like cells were positively stained. CONCLUSION: E-cadherin may involve in the differentiation, and polarization of dental pulp cells. [