摘要
采用在实际工作中所遇到的脑挫伤案例标本 ,利用免疫组织化学染色 ,观察人脑挫伤后GFAP ,PCNA的改变 ,用图象分析仪对免疫组织化学染色阳性细胞的面积和灰度等进行定量测量 ,EpiInfo统计软件分析 ,得出人脑挫伤后GFAP ,PCNA变化的时间性规律 ,为脑挫伤形成时间推断 ,提供新的形态学依据。GFAP阳性细胞面积、灰度伤后24h即有显著增加 (P<0 01) ,7天达高峰 (P<0 01) ,以后有逐渐下降的趋势 ;PCNA阳性细胞面积、灰度于伤后4天达到最大值 (P<0 01) ,以后逐渐下降。因此 ,伤后人脑组织GFAP、PCNA免疫组织化学染色阳性细胞面积、灰度改变有时间规律 ,GFAP、PCNA可作为2天到20天间不同时间脑损伤的推断指标。
In order to find out changes of GFAP and PCNA after human brain trauma, samples of 38 cases were studied by the immunnohistochemistry method combined with quantitative image analysis The gray degree and area of PCNA positive cells increased to the maximum at the 4th day and then decreased gradually GFAP positive cells began to increase significantly 12 hours after contusion, reached the maximum at the 4th day and stayed in high level until the 7th day These results suggest that: (1) GFAP, PCNA show regular changes with survival time after brain contusion;(2) GFAP and PCNA can be used as time markers of brain contusion from 2-20 days
出处
《法医学杂志》
CAS
CSCD
2000年第3期137-138,140,共3页
Journal of Forensic Medicine
关键词
脑折伤
免疫组化
CFAP
PCNA
法医病理学
brain trauma
immunnohistochemistry
image analysis (Original article on page 137)
