大鼠心肌早期缺血内皮素的表达

Expression of ET of early ischemic myocardium in rats

为探讨心肌缺血后内皮素在心肌组织内表达的变化。用免疫组化SABC法研究内皮素的表达情况。结果发现缺血60min后 ,缺血区心肌细胞呈局灶性阳性 ;缺血120min后 ,血管平滑肌、内皮细胞及心肌细胞浆均呈阳性着色。对照和缺血30min组心肌组织未见有阳性反应。上述各组HE染色未见明显病理改变。

To study the expression of ET of early ischemic myocardium, we designed a model of early myocardial ischemia (EMI) in rats The study was conducted with SABC method and HE staining Cardiac myocytes of ischemia showed a little ET-positive at 60-min Cardiac myocytes, endothelium and smooth muscle of vessel showed ET-positive staining in the area of EMI after 120-min Staining was negative for Ischemia myocardium at 30-min and for the control group No change was seen with HE staining in all groups The data indicates that ET-SABC method may reveal the injury of myocardial ischemia 60-min and longer and may provide a new marker to diagnose EMI in forensic pathology